Rhesus macaque CD155/PVR Protein (CD1-CM355)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp28-Asn343
Catalog #CD1-CM355
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Product Description
 Recombinant Rhesus macaque CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp28-Asn343.
Product Background
 CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
Product Category
 Recombinant Protein
Target
 CD155/PVR
Synonyms
 CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession
Species
 Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Asp28-Asn343
Molecular Weight
 The protein has a predicted MW of 35.5 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 uM as determined in SPR assay (Biacore T200).
Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test).
Rhesus macaque CD155/PVR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque CD155/PVR is greater than 95% as determined by SEC-HPLC.

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