Rhesus macaque CD155/PVR Protein (CD1-CM355)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp28-Asn343
Catalog #CD1-CM355
Regular price $346.50
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Product Description

Recombinant Rhesus macaque CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp28-Asn343.

Product Background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

CD155/PVR

Synonyms

CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946

Accession

Species

Rhesus macaque

Biotinylated

no

Amino Acid Range

Asp28-Asn343

Molecular Weight

The protein has a predicted MW of 35.5 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 uM as determined in SPR assay (Biacore T200).
Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test). Immobilized Rhesus macaque CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test).
Rhesus macaque CD155/PVR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rhesus macaque CD155/PVR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque CD155/PVR is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque CD155/PVR is greater than 95% as determined by SEC-HPLC.

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