Rhesus macaque CD4/LEU3 Protein (CD4-RM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Rhesus macaque CD4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys26-Trp390

Catalog: CD4-RM101

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Product Description	Recombinant Rhesus macaque CD4/LEU3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys26-Trp390.
Product Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category	Recombinant Protein

Target	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession	G7N5T8
Species	Rhesus macaque
Amino Acid Range	Lys26-Trp390
Molecular Weight	The protein has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Rhesus macaque CD4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Rhesus macaque CD4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.
	The purity of Rhesus macaque CD4 is greater than 95% as determined by SEC-HPLC.
	Rhesus macaque CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.