- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
- Activity: Immobilized Rhesus macaque CD4, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 13.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Lys26-Trp390
Product Description |
Recombinant Rhesus macaque CD4/LEU3 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Lys26-Trp390. |
Product Background |
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule: peptide complex. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
CD4/LEU3 |
Synonyms |
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Lys26-Trp390 |
Molecular Weight |
The protein has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rhesus macaque CD4, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 13.0 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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