• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque CDCP1 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Phe30-Thr667
Catalog #CDC-CM101-UL
Price
Regular price $41745
Regular price Sale price $41745
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Size
Avantor VWR Fisher Scientific

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Rhesus macaque CDCP1 Protein, Ultra Low Endotoxin (CDC-CM101-UL) Summary

Product Description

Recombinant Rhesus macaque CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Thr667.

Product Background

Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Rhesus macaque CDCP1 Protein, Ultra Low Endotoxin (CDC-CM101-UL) Specifications/Details

Protein
CDCP1
Synonyms
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession
Species
Rhesus macaque
Biotinylated
no
Amino Acid Range
Phe30-Thr667
Molecular Weight
The protein has a predicted MW of 73.2 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rhesus macaque CDCP1 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
Product Tag
C-His
Fluorophore
Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rhesus macaque CDCP1 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). Immobilized Rhesus macaque CDCP1 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
Rhesus macaque CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. Rhesus macaque CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Rhesus macaque CDCP1, His Tag with an affinity constant of 80.70 nM as determined in BLI assay . Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Rhesus macaque CDCP1, His Tag with an affinity constant of 80.70 nM as determined in BLI assay .
The purity of Rhesus macaque CDCP1 is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque CDCP1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...