Rhesus macaque Complement Factor D/CFD Protein (CFD-CM101)
Rhesus macaque Complement Factor D/CFD Protein (CFD-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
  • Amino Acid Range: Gln21-Ala253
Catalog #CFD-CM101
Regular price $346.50
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Rhesus macaque Complement Factor D/CFD Protein (CFD-CM101) Summary

Product Description

Recombinant Rhesus macaque Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Ala253.

Product Background

Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Rhesus macaque Complement Factor D/CFD Protein (CFD-CM101) Specifications/Details

Protein
Complement Factor D/CFD
Synonyms
Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46
Accession
Species
Rhesus macaque
Biotinylated
no
Amino Acid Range
Gln21-Ala253
Molecular Weight
The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA. Immobilized Rhesus macaque Complement Factor D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
Rhesus macaque Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rhesus macaque Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque Complement Factor D is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque Complement Factor D is greater than 95% as determined by SEC-HPLC.
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