Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein (HLG-RM41C)
Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein (HLG-RM41C)
Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein (HLG-RM41C)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4ug/ml determined by ELISA (QC Test). The affinity constant of 27.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Catalog #HLG-RM41C
Regular price $310.00
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Product Description
Recombinant Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.
Product Background
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Product Category
Recombinant Protein
Target
HLA-G&B2M&Peptide (RIIPRHLQL) Monomer
MHC Form
Monomer
Allele
HLA-G
Accession
Species
Rhesus macaque
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Molecular Weight
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4ug/ml determined by ELISA (QC Test). The affinity constant of 27.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 27.18 nM as determined in SPR assay (Biacore T200).
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4ug/ml determined by ELISA (QC Test).
Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.

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