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Rhesus macaque IL-18 R1/CD218a Protein, Ultra Low Endotoxin (IL8-RM2R1-UL) Summary
Product Description |
Recombinant Rhesus macaque IL-18 R1/CD218a Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Cys22-Gly329. |
Product Background |
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Rhesus macaque IL-18 R1/CD218a Protein, Ultra Low Endotoxin (IL8-RM2R1-UL) Specifications/Details
Protein |
IL-18 R1/CD218a |
Synonyms |
CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Cys22-Gly329 |
Molecular Weight |
The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.71 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Rhesus macaque IL-18 R1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-18, No Tag with an affinity constant of 0.71 nM as determined in SPR assay (Biacore T200) (QC Test). |
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Rhesus macaque IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Rhesus macaque IL-18 R1, hFc Tag on ProA-Biosensor, can bind Human IL-18, No Tag with an affinity constant of 2.01 nM as determined in BLI assay (Gator). |
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The purity of Rhesus macaque IL-18 R1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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