• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for h11B6, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. The affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: /
Catalog #KLK-CM102
Price
Regular price $67500
Regular price Sale price $67500
/
Size
Avantor VWR Fisher Scientific

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Rhesus macaque Kallikrein 2/KLK2 Protein (KLK-CM102) Summary

Product Description

 Recombinant Rhesus macaque Kallikrein 2/KLK2 Protein is expressed from HEK293 with His tag at the C-terminus.

Product Background

 Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.

Rhesus macaque Kallikrein 2/KLK2 Protein (KLK-CM102) Specifications/Details

Protein
 Kallikrein 2/KLK2
Synonyms
 KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2;hGK1
Accession
Species
 Rhesus macaque
Biotinylated
 no
Amino Acid Range
 /
Molecular Weight
 The protein has a predicted MW of 27.63 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under Non-reduced condition based on Bis-Tris PAGE result.
Activity
 Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for h11B6, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. The affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 7.5).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

h11B6 captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200). h11B6 captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
Pasritamig captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 34.85 nM as determined in SPR assay (Biacore T200). Pasritamig captured on CM5 Chip via Protein A can bind Rhesus macaque Kallikrein 2, His Tag with an affinity constant of 34.85 nM as determined in SPR assay (Biacore T200).
Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for h11B6, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for h11B6, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Pasritamig with the EC50 of 12.5ng/ml determined by ELISA. Immobilized Rhesus macaque Kallikrein 2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Pasritamig with the EC50 of 12.5ng/ml determined by ELISA.
Rhesus macaque Kallikrein 2 on Bis-Tris PAGE under Non-reduced condition. The purity is greater than 95%. Rhesus macaque Kallikrein 2 on Bis-Tris PAGE under Non-reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque Kallikrein 2 is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque Kallikrein 2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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