Regular price
$297.00
Order by 4pm ET for delivery on:
Rhesus macaque LILRB1/CD85j/ILT2 Protein (LIL-CM2B1) Summary
Product Description |
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser17-His456. |
Product Background |
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Rhesus macaque LILRB1/CD85j/ILT2 Protein (LIL-CM2B1) Specifications/Details
Protein |
LILRB1/CD85j/ILT2 |
Synonyms |
ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
Accession |
|
Species |
Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Ser17-His456 |
Molecular Weight |
The protein has a predicted MW of 74.5 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 51.44 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 51.44 nM as determined in SPR assay (Biacore T200). |
|
|
Rhesus macaque LILRB1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
