HRSV (B, strain 18537) Post-fusion glycoprotein F0 Protein (RSV-VM1PB)
Catalog: RSV-VM1PB
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| Product Description | Recombinant HRSV (B, strain 18537) Post-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus. |
Product Background |
Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body. |
Product Category |
Recombinant Protein |
Target |
Post-fusion glycoprotein F0 |
Species |
HRSV (B, strain 18537) |
| Molecular Weight | The protein has a predicted MW of 55.96 kDa. The protein migrates to 18-25 kDa and 42-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized HRSV (B, strain 18537) Post-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 3.4ng/ml and not bind Anti-Fusion glycoprotein F0 (D25) Antibody determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized HRSV (B, strain 18537) Post-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 3.4ng/ml and not bind Anti-Fusion glycoprotein F0 (D25) Antibody determined by ELISA. |
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HRSV (B, strain 18537) Post-fusion glycoprotein F0 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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