RSV (B) Post-fusion glycoprotein F0 Protein (RSV-VM1PB)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized RSV (B) Post-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 3.4ng/ml and not bind Anti-Fusion glycoprotein F0 (D25) Antibody determined by ELISA. See testing image for detail.

Catalog: RSV-VM1PB

$675.00
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Product Description Recombinant RSV (B) Post-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.

Product Background

Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.

Product Category

Recombinant Protein

Target

Post-fusion glycoprotein F0

Species

RSV
Molecular Weight The protein has a predicted MW of 55.96 kDa. The protein migrates to 18-25 kDa and 42-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized RSV (B) Post-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 3.4ng/ml and not bind Anti-Fusion glycoprotein F0 (D25) Antibody determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized RSV (B) Post-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 3.4ng/ml and not bind Anti-Fusion glycoprotein F0 (D25) Antibody determined by ELISA.
RSV (B) Post-fusion glycoprotein F0 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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