HRSV (B, strain 18537) Pre-fusion glycoprotein F0 Protein (RSV-VM1RB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized HRSV (B, strain 18537) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
Catalog #RSV-VM1RB
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Product Description
 Recombinant HRSV (B, strain 18537) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.
Product Background
 Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.
Product Category
 Recombinant Protein
Target
 Pre-fusion glycoprotein F0
Species
 HRSV (B, strain 18537)
Biotinylated
 no
Molecular Weight
 The protein has a predicted MW of 56.50 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized HRSV (B, strain 18537) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized HRSV (B, strain 18537) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
Immobilized Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for HRSV (B, strain 18537) Pre-fusion glycoprotein F0, His Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test).
HRSV (B, strain 18537) Pre-fusion glycoprotein F0 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of HRSV (B, strain 18537) Pre-fusion glycoprotein F0 is greater than 95% as determined by SEC-HPLC.
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