RSV (B) Pre-fusion glycoprotein F0 Protein (RSV-VM1RB)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized RSV (B) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Catalog: RSV-VM1RB

$675.00
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Product Description Recombinant RSV (B) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.

Product Background

Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.

Product Category

Recombinant Protein

Target

Pre-fusion glycoprotein F0

Species

RSV
Molecular Weight The protein has a predicted MW of 56.50 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized RSV (B) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized RSV (B) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Immobilized Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for RSV (B) Pre-fusion glycoprotein F0, His Tag with the EC50 of 13.6ng/ml determined by ELISA.
The purity of RSV (B) Pre-fusion glycoprotein F0 is greater than 95% as determined by SEC-HPLC.
RSV (B) Pre-fusion glycoprotein F0 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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