• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Measured by its ability to cleave a peptide substrate, MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2.The specific activity is > 5 pmol/min/ug.
  • Amino Acid Range: Ser1-Gln306
Catalog #COV-VE0CL
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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SARS-CoV-2 3CLpro/3C-like Protease Protein (COV-VE0CL) Summary

Product Description

 Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein is expressed from E.coli without tag.It contains Ser1-Gln306.

Product Background

 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

SARS-CoV-2 3CLpro/3C-like Protease Protein (COV-VE0CL) Specifications/Details

Protein
 3CLpro/3C-like Protease
Synonyms
 3C-like protease; 3CL Protease; 3CL-Mpro; 3CL Pro; COVID-19; M Proteinase; Mpro;COVID19
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Ser1-Gln306
Molecular Weight
 The protein has a predicted MW of 33.8 kDa same as Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave a peptide substrate, MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2.The specific activity is > 5 pmol/min/ug.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

SARS-CoV-2 3CLpro on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-CoV-2 3CLpro on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...