Product Description |
Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (F140A) is expressed from E.coli without tag.It contains Ser1-Gln306(F140A). |
Product Background |
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. |
Product Category |
Recombinant Protein |
Target |
3CLpro/3C-like Protease |
Synonyms |
3CL Protease; 3CL-Mpro; M Proteinase; Mpro |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Ser1-Gln306(F140A) |
Molecular Weight |
The protein has a predicted MW of 33.72 kDa same as Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
SARS-CoV-2 3CLpro (F140A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of SARS-CoV-2 3CLpro (F140A) is greater than 95% as determined by SEC-HPLC. |
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