SARS-COV-2 NSP7&NSP8 Protein (NSP-VE178) Summary
Product Description |
SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8). |
Product Background |
The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
SARS-COV-2 NSP7&NSP8 Protein (NSP-VE178) Specifications/Details
Protein |
NSP7&NSP8 |
Synonyms |
SP7&NSP8; nsp7&nsp8 |
Accession |
|
Species |
SARS-CoV-2 |
Biotinylated |
no |
Amino Acid Range |
Ser1-Gln83(NSP7) & Ala1-Gln198(NSP8) |
Molecular Weight |
The protein has a predicted MW of 32.8 kDa same as Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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