Product Description |
Recombinant SARS-Cov-2 Spike RBD(B.1.640.2/IHU) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y). |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein |
Protein |
Spike RBD (B.1.640.2/IHU) |
Synonyms |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y) |
Molecular Weight |
The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. |
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SARS-Cov-2 Spike RBD (B.1.640.2/IHU) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS-Cov-2 Spike RBD (B.1.640.2/IHU) is greater than 95% as determined by SEC-HPLC. |
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