SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) Protein (GP1-VM1BD)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Phe541(K417T, E484K, N501Y)
Catalog #GP1-VM1BD
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Product Description
 Recombinant SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(K417T, E484K, N501Y).
Product Background
 The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category
 Recombinant Protein
Target
 Spike RBD (Gamma P.1/P.1.1/P.1.2)
Synonyms
 S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Arg319-Phe541(K417T, E484K, N501Y)
Molecular Weight
 The protein has a predicted MW of 26.21 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.7ng/ml determined by ELISA.
SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 Spike RBD (Gamma P.1/P.1.1/P.1.2) is greater than 95% as determined by SEC-HPLC.

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