SARS-CoV-2 Spike RBD (Lambda C.37) Protein (LCD-VM1BD)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg319-Phe541(L452Q, F490S)

Catalog: LCD-VM1BD

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Product Description Recombinant SARS-CoV-2 Spike RBD (Lambda C.37) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(L452Q, F490S).

Product Background

The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

Product Category

Recombinant Protein

Target

Spike RBD (Lambda C.37)

Gene/Protein ID

QHD43416.1

Species

SARS-COV-2

Exact Sequence

Arg319-Phe541(L452Q, F490S)
Molecular Weight The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Activity

Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC.
SARS-CoV-2 Spike RBD (Lambda C.37) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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