Name: SARS-CoV-2 Spike RBD (Lambda C.37) Protein (LCD-VM1BD)
Brand: KactusBio
SKU: LCD-VM1BD-100UG
Price: 375.0 USD
Availability: InStock

SARS-CoV-2 Spike RBD (Lambda C.37) Protein (LCD-VM1BD)

Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.

SARS-CoV-2 Spike RBD (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg319-Phe541(L452Q, F490S)

Catalog #LCD-VM1BD

Size

100UG

500UG

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Product Description	Recombinant SARS-CoV-2 Spike RBD (Lambda C.37) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(L452Q, F490S).
Product Background	The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.
Product Category	Recombinant Protein / Antibody discovery

Protein	Spike RBD (Lambda C.37)
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Arg319-Phe541(L452Q, F490S)
Molecular Weight	The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
	SARS-CoV-2 Spike RBD (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC.