SARS-COV-2 Spike RBD (N501Y) Protein (COV-VM1BY)
Catalog: COV-VM1BY
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| Product Description | Recombinant SARS-COV-2 Spike RBD (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y). |
Product Background |
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. |
Product Category |
Recombinant Protein |
Target |
Spike RBD (N501Y) |
| Synonyms | S protein RBD; S glycoprotein RBD; Spike protein RBD |
Gene/Protein ID |
QHD43416.1 |
Species |
SARS-COV-2 |
Exact Sequence |
Arg319-Phe541(N501Y) |
| Molecular Weight | The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.74nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human ACE2 captured on Protein A chip, can bind SARS-COV-2 Spike RBD (N501Y) , His Tag with an affinity constant of 1.74nM as determined in a SPR assay (Biacore T200). |
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Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). |
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The purity of SARS-COV-2 Spike RBD (N501Y) is greater than 95% as determined by SEC-HPLC. |
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SARS-COV-2 Spike RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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