SARS-COV-2 Spike RBD (N501Y) Protein (COV-VM1BY)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.74nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Arg319-Phe541(N501Y)
Catalog #COV-VM1BY
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Product Description
 Recombinant SARS-COV-2 Spike RBD (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y).
Product Background
 The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.
Product Category
 Recombinant Protein
Target
 Spike RBD (N501Y)
Synonyms
 S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Arg319-Phe541(N501Y)
Molecular Weight
 The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.74nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ACE2 captured on Protein A chip, can bind SARS-COV-2 Spike RBD (N501Y) , His Tag with an affinity constant of 1.74nM as determined in a SPR assay (Biacore T200).
Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).
SARS-COV-2 Spike RBD (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike RBD (N501Y) is greater than 95% as determined by SEC-HPLC.

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