SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) Protein (BA2-VM121)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.12.1) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)

Catalog: BA2-VM121

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Product Description Recombinant SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H).
Product Category Recombinant Protein
Target Spike RBD (Omicron BA.2.12.1)
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)
Molecular Weight The protein has a predicted MW of 26.09 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.12.1) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.12.1) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
The purity of SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) is greater than 95% as determined by SEC-HPLC.
SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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