SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) Protein (BA2-VM121)
Catalog: BA2-VM121
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Product Description | Recombinant SARS-Cov-2 Spike RBD (Omicron BA.2.12.1) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H). |
Product Category | Recombinant Protein |
Target | Spike RBD (Omicron BA.2.12.1) |
Gene/Protein ID | QHD43416.1 |
Species | SARS-COV-2 |
Exact Sequence | Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H) |
Molecular Weight | The protein has a predicted MW of 26.09 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.12.1) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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