SARS-CoV-2 Spike RBD (Omicron BA.2.74) Protein (BA2-HM174)
SARS-CoV-2 Spike RBD (Omicron BA.2.74) Protein (BA2-HM174)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.74) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Phe541(T19I, del24-26, A27S, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452M, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)
Catalog #BA2-HM174
Regular price $345.00
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Product Description
Recombinant SARS-CoV-2 Spike RBD (Omicron BA.2.74) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(T19I, del24-26, A27S, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452M, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H).
Product Background
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.
Product Category
Recombinant Protein
Target
Spike RBD (Omicron BA.2.74)
Accession
Species
SARS-COV-2
Biotinylated
no
Amino Acid Range
Arg319-Phe541(T19I, del24-26, A27S, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452M, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)
Molecular Weight
The protein has a predicted MW of 26.04 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result.
Activity
Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.74) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.74) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
SARS-CoV-2 Spike RBD (Omicron BA.2.74) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 Spike RBD (Omicron BA.2.74) is greater than 95% as determined by SEC-HPLC.

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