SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) Protein (BF7-HM146)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Phe541(G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H)

Catalog: BF7-HM146

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Product Description Recombinant SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H).

Product Background

 The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

Product Category

 Recombinant Protein

Target

 Spike RBD (Omicron BF.7/BA.4.6)
Accession

Species

 SARS-COV-2

Amino Acid Range

 Arg319-Phe541(G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H)
Molecular Weight The protein has a predicted MW of 25.99 kDa. Due to glycosylation, the protein migrates to 35-43 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
The purity of SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) is greater than 95% as determined by SEC-HPLC.
SARS-CoV-2 Spike RBD (Omicron BF.7/BA.4.6) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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