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SARS-CoV-2 Spike RBD (Omicron BQ.1.1) Protein, Ultra Low Endotoxin (BQ1-HM111-UL) Summary
Product Description |
Recombinant SARS-CoV-2 Spike RBD (Omicron BQ.1.1) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H). |
Product Background |
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
SARS-CoV-2 Spike RBD (Omicron BQ.1.1) Protein, Ultra Low Endotoxin (BQ1-HM111-UL) Specifications/Details
Protein |
Spike RBD |
Synonyms |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Arg319-Phe541(G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H) |
Molecular Weight |
The protein has a predicted MW of 25.98 kDa. Due to glycosylation, the protein migrates to 35-43 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-CoV-2 Spike RBD (Omicron BQ.1.1) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized SARS-CoV-2 Spike RBD (Omicron BQ.1.1) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. |
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|
SARS-CoV-2 Spike RBD (Omicron BQ.1.1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS-CoV-2 Spike RBD (Omicron BQ.1.1) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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