Product Description |
Recombinant SARS-CoV-2 Spike RBD (Omicron XBB.1.5) Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Arg319-Phe541 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H). |
Product Background |
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. |
Product Category |
Recombinant Protein |
Target |
Spike RBD (Omicron XBB.1.5) |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Arg319-Phe541 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H) |
Molecular Weight |
The protein has a predicted MW of 26.22 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-Cov-2 Spike RBD (Omicron XBB.1.5) , His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-Cov-2 Spike RBD (Omicron XBB.1.5) , His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA. |
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SARS-CoV-2 Spike RBD (Omicron XBB.1.5) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS-CoV-2 Spike RBD (Omicron XBB.1.5) is greater than 95% as determined by SEC-HPLC. |
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