SARS-COV-2 Spike RBD Protein (COV-VM2BD)
SARS-COV-2 Spike RBD Protein (COV-VM2BD)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Phe541
Catalog #COV-VM2BD
Regular price $330.00
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Product Description

Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Arg319-Phe541.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

Spike RBD

Synonyms

S protein RBD; S glycoprotein RBD; Spike protein RBD

Accession

Species

SARS-COV-2

Biotinylated

no

Amino Acid Range

Arg319-Phe541

Molecular Weight

The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.

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