Name: SARS-COV-2 Spike RBD Protein (COV-VM4BD)
Brand: KactusBio
SKU: COV-VM4BD-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Arg319-Asn532

Catalog #COV-VM4BD

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg319-Asn532.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery

Protein	Spike RBD
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Arg319-Asn532
Molecular Weight	The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ACE2, hFc Tag captured on Protein A chip, can bind SARS-COV-2 Spike RBD, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200).
	Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
	SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.