SARS-COV-2 Spike RBD Protein (COV-VM4BD)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Arg319-Asn532

Catalog: COV-VM4BD

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Product Description Recombinant SARS-COV-2 Spike RBD Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Arg319-Asn532.
Product Category Recombinant Protein
Target Spike RBD
Synonyms S protein RBD; S glycoprotein RBD; Spike protein RBD
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Arg319-Asn532
Molecular Weight The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike RBD, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ACE2, hFc Tag captured on Protein A chip, can bind SARS-COV-2 Spike RBD, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200).
Immobilized SARS-COV-2 Spike RBD, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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