Name: SARS-COV-2 Spike RBD Protein (COV-VM4BD)
Brand: KactusBio
SKU: COV-VM4BD-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Arg319-Asn532

Catalog #COV-VM4BD

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg319-Asn532.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Spike RBD
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Arg319-Asn532
Molecular Weight	The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ACE2, hFc Tag captured on Protein A chip, can bind SARS-COV-2 Spike RBD, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200).
	Immobilized SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
	SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.