SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM1FO)
Catalog: COV-VM1FO
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Product Description | Recombinant SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein |
Target |
Spike S (Omicron B.1.1.529) Trimer |
Synonyms | S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
Gene/Protein ID |
QHO60594.1 |
Species |
SARS-COV-2 |
Exact Sequence |
Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) |
Molecular Weight | The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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