SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 61.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Catalog: IHU-VM1ST

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Product Description Recombinant SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein is expressed from Expi293 with His tag at the C-terminal.It contains Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Product Category Recombinant Protein
Target Spike S (B.1.640.2/IHU) Trimer
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Molecular Weight The protein has a predicted MW of 133.60 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 61.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 61.8ng/ml determined by ELISA.
SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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