Name: SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)
Brand: KactusBio
SKU: IHU-VM1ST-100UG
Price: 412.5 USD
Availability: InStock

SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)

Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).

SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Catalog #IHU-VM1ST

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery

Protein	Spike S (B.1.640.2/IHU) Trimer
Synonyms	S protein; Spike glycoprotein; S glycoprotein; COVID-19
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Molecular Weight	The protein has a predicted MW of 133.60 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).
	SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.