SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein (IHU-VM1ST)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 89.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Catalog: IHU-VM1ST

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Product Description Recombinant SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Background

 The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

 Recombinant Protein

Target

 Spike S (B.1.640.2/IHU) Trimer

Gene/Protein ID

 QHD43416.1

Species

 SARS-COV-2

Exact Sequence

 Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Molecular Weight The protein has a predicted MW of 133.60 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 89.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 89.9ng/ml determined by ELISA.
SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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