SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)
Catalog: COV-VM4LG
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| Product Description | Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G). |
Product Background |
The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin. |
Product Category |
Recombinant Protein |
Target |
Spike S Trimer (D614G) |
| Synonyms | S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
Gene/Protein ID |
QHD43416.1 |
Species |
SARS-COV-2 |
Exact Sequence |
Val16-Glu1188(D614G) |
| Molecular Weight | The protein has a predicted MW of 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. |
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The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC. |
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SARS-COV-2 Spike S Trimer (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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