SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)
SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)
SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Glu1188(D614G)
Catalog #COV-VM4LG
Regular price $300.00
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Product Description
Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G).
Product Background
The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.
Product Category
Recombinant Protein
Target
Spike S Trimer (D614G)
Synonyms
S protein; Spike glycoprotein; S glycoprotein; COVID-19
Accession
Species
SARS-COV-2
Biotinylated
no
Amino Acid Range
Val16-Glu1188(D614G)
Molecular Weight
The protein has a predicted MW of 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Bis-Tris PAGE result.
Activity
Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
SARS-COV-2 Spike S Trimer (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC.

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