SARS-COV-2 Spike S Trimer Protein (COV-VM2SS)

  • Express System: HEK293
  • Product Tag: C-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Glu1188

Catalog: COV-VM2SS

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Product Description Recombinant SARS-COV-2 Spike S Trimer Protein is expressed from HEK293 with monomeric hFc tag at the C-Terminus.It contains Val16-Glu1188.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein

Target

Spike S Trimer
Synonyms S protein; Spike glycoprotein; S glycoprotein; COVID-19
Accession

Species

SARS-COV-2

Amino Acid Range

Val16-Glu1188
Molecular Weight The protein has a predicted MW of 158.3 kDa. Due to glycosylation, the protein migrates to 230-250 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-monomeric hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA.
The purity of SARS-COV-2 Spike S (Trimer) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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