ELISA binding curve showing interaction between SARS-CoV-2 Spike S (Trimer), hFc tag and human ACE2 protein.
SARS-COV-2 Spike S Trimer Protein (COV-VM2SS)
  • Express System: HEK293
  • Product Tag: C-monomeric hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Glu1188
Catalog #COV-VM2SS
Regular price $412.50
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Product Description

Recombinant SARS-COV-2 Spike S Trimer Protein is expressed from HEK293 with monomeric hFc tag at the C-Terminus.It contains Val16-Glu1188 trimer design.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Spike S Trimer

Synonyms

S protein; Spike glycoprotein; S glycoprotein; COVID-19

Accession

Species

SARS-COV-2

Biotinylated

no

Amino Acid Range

Val16-Glu1188

Molecular Weight

The protein has a predicted MW of 158.3 kDa. Due to glycosylation, the protein migrates to 230-250 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-monomeric hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S (Trimer) , hFc Tag with the EC50 of 0.22ug/ml determined by ELISA.
SARS-COV-2 Spike S (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike S (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S (Trimer) is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike S (Trimer) is greater than 95% as determined by SEC-HPLC.

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