SARS-COV-2 Spike S Trimer Protein (COV-VM4SS)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Glu1188

Catalog: COV-VM4SS

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Product Description Recombinant SARS-COV-2 Spike S is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Val16-Glu1188.
Product Category Recombinant Protein
Target Spike S Trimer
Synonyms S protein; Spike glycoprotein; S glycoprotein; COVID-19
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Val16-Glu1188
Molecular Weight The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA.
Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 47.3/37.8/37.0ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike S Trimer is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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