SARS-COV-2 Spike S Trimer Protein (COV-VM4SS)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized SARS-COV-2 Spike S Trimer, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Glu1188

Catalog: COV-VM4SS

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Product Description Recombinant SARS-COV-2 Spike S is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein

Target

Spike S Trimer
Synonyms S protein; Spike glycoprotein; S glycoprotein; COVID-19

Gene/Protein ID

QHD43416.1

Species

SARS-COV-2

Exact Sequence

Val16-Glu1188
Molecular Weight The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.

Activity

Immobilized SARS-COV-2 Spike S Trimer, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S Trimer, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.
SARS-COV-2 Spike S Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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