SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein (COV-VM1SO)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)

Catalog: COV-VM1SO

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Product Description Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H).

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein

Target

Spike S1 (Omicron B.1.1.529)
Synonyms S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Gene/Protein ID

QHO60594.1

Species

SARS-COV-2

Exact Sequence

Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)
Molecular Weight The protein has a predicted MW of 76.38 kDa. Due to glycosylation, the protein migrates to 130-145 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ACE2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ACE2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8ug/ml determined by ELISA.
The purity of SARS-COV-2 Spike S1 (Omicron B.1.1.529) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S1 (Omicron B.1.1.529) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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