SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein (DB1-VM1S1)
Catalog: DB1-VM1S1
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Product Description | Recombinant SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R). |
Product Category | Recombinant Protein |
Target | Spike S1 (Delta B.1.617.2) |
Gene/Protein ID | QHD43416.1 |
Species | SARS-COV-2 |
Exact Sequence | Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R) |
Molecular Weight | The protein has a predicted MW of 75.92 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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