SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein (DB1-VM1S1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R)

Catalog: DB1-VM1S1

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Product Description Recombinant SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R).
Product Category Recombinant Protein
Target Spike S1 (Delta B.1.617.2)
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R)
Molecular Weight The protein has a predicted MW of 75.92 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
The purity of SARS-CoV-2 Spike S1 (Delta B.1.617.2) is greater than 95% as determined by SEC-HPLC.
SARS-CoV-2 Spike S1 (Delta B.1.617.2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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