SARS-COV-2 Spike S1 (Deltacron) Protein (COV-VM1SD)
Catalog: COV-VM1SD
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Product Description | Recombinant SARS-COV-2 Spike S1(Deltacron) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Alanine substitutions (R683A) are introduced to stabilize the trimeric prefusion state of SARS-COV-2 Spike S1(Deltacron) Protein and abolish the furin cleavage site, respectively. |
Product Category | Recombinant Protein |
Target | Spike S1 (Deltacron) |
Gene/Protein ID | QHD43416.1 |
Species | SARS-COV-2 |
Exact Sequence | Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H) |
Molecular Weight | The protein has a predicted MW of 73.94 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Liquid |
Shipping | Shipped with blue ice. |
Formulation | Supplied as 0.22μm filtered solution in PBS (pH 7.4). |
Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
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