SARS-COV-2 Spike S1 (Deltacron) Protein (COV-VM1SD)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)

Catalog: COV-VM1SD

$469.00
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Product Description Recombinant SARS-COV-2 Spike S1(Deltacron) Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Alanine substitutions (R683A) are introduced to stabilize the trimeric prefusion state of SARS-COV-2 Spike S1(Deltacron) Protein and abolish the furin cleavage site, respectively.
Product Category Recombinant Protein
Target Spike S1 (Deltacron)
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)
Molecular Weight The protein has a predicted MW of 73.94 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml determined by ELISA.
SARS-COV-2 Spike S1 (Deltacron) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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