SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein (COV-VM1SM)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Arg685(N501Y,K417N,E484K)
Catalog #COV-VM1SM
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Product Description
 Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).
Product Background
 The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category
 Recombinant Protein
Target
 Spike S1 (N501Y,K417N,E484K)
Synonyms
 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Val16-Arg685(N501Y,K417N,E484K)
Molecular Weight
 The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA.
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.

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