SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein (COV-VM1SM)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Arg685(N501Y,K417N,E484K)
Catalog #COV-VM1SM
Regular price $346.50
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Product Description

Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

Spike S1 (N501Y,K417N,E484K)

Synonyms

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Accession

Species

SARS-COV-2

Biotinylated

no

Amino Acid Range

Val16-Arg685(N501Y,K417N,E484K)

Molecular Weight

The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.

Activity

Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA. Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02ug/ml determined by ELISA.
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.

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