Product Description |
Recombinant SARS-CoV-2 Spike S1 (Omicron BA.2.12.1) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19I, L24S, PPA25-27 del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H). |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein |
Target |
Spike S1 (Omicron BA.2.12.1) |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Gln14-Arg683(T19I, L24S, PPA25-27 del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H) |
Molecular Weight |
The protein has a predicted MW of 76.05 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS-CoV-2 Spike S1 (Omicron BA.2.12.1) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-CoV-2 Spike S1 (Omicron BA.2.12.1) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA. |
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SARS-CoV-2 Spike S1 (Omicron BA.2.12.1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS-CoV-2 Spike S1 (Omicron BA.2.12.1) is greater than 95% as determined by SEC-HPLC. |
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