SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (BAS-VM145)
Catalog: BAS-VM145
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| Product Description | Recombinant SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H). |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein |
Target |
Spike S1 (Omicron BA.4/BA.5/BA.5.2) |
Gene/Protein ID |
QHD43416.1 |
Species |
SARS-COV-2 |
Exact Sequence |
Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H) |
| Molecular Weight | The protein has a predicted MW of 75.76 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.4ng/ml determined by ELISA. |
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The purity of SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) is greater than 95% as determined by SEC-HPLC. |
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SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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