SARS-COV-2 Spike S1 Protein (COV-VM2S1)
SARS-COV-2 Spike S1 Protein (COV-VM2S1)
SARS-COV-2 Spike S1 Protein (COV-VM2S1)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val16-Arg685
Catalog #COV-VM2S1
Regular price $379.50
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Product Description

Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val16-Arg685.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Spike S1

Synonyms

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Accession

Species

SARS-COV-2

Biotinylated

no

Amino Acid Range

Val16-Arg685

Molecular Weight

The protein has a predicted MW of 101.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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