Name: SARS-COV-2 Spike S1 Protein (COV-VM2S1)
Brand: KactusBio
SKU: COV-VM2S1-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val16-Arg685

Catalog #COV-VM2S1

Size

100UG

500UG

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Product Description	Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val16-Arg685.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Spike S1
Synonyms	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession	QHO60594.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Val16-Arg685
Molecular Weight	The protein has a predicted MW of 101.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
	SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.