• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val16-Arg685
Catalog #COV-VM2S1
Price
Regular price $379.50
Regular price Sale price $379.50
/
Size
Avantor VWR Fisher Scientific

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SARS-COV-2 Spike S1 Protein (COV-VM2S1) Summary

Product Description

 Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val16-Arg685.

Product Background

 The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

SARS-COV-2 Spike S1 Protein (COV-VM2S1) Specifications/Details

Protein
 Spike S1
Synonyms
 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Val16-Arg685
Molecular Weight
 The protein has a predicted MW of 101.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200). Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 uM as determined in SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...