SARS Spike RBD Protein (SAR-VM4BD)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg306-Phe527
Catalog #SAR-VM4BD
Regular price $300.00
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Product Description
Recombinant SARS Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527.
Product Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category
Recombinant Protein
Target
Spike RBD
Synonyms
S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
SARS
Biotinylated
no
Amino Acid Range
Arg306-Phe527
Molecular Weight
The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Bis-Tris PAGE result.
Activity
Immobilized SARS Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA.
SARS Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS Spike RBD is greater than 95% as determined by SEC-HPLC.

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