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Non-biotinylated SARS Spike RBD Protein, Ultra Low Endotoxin (SAR-VM4BD-UL) Summary
Product Description |
Recombinant SARS Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527. |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated SARS Spike RBD Protein, Ultra Low Endotoxin (SAR-VM4BD-UL) Specifications/Details
Protein |
Spike RBD |
Synonyms |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
Accession |
|
Species |
SARS |
Biotinylated |
no |
Amino Acid Range |
Arg306-Phe527 |
Molecular Weight |
The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized SARS Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized SARS Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA. |
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SARS Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of SARS Spike RBD is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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