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Non-biotinylated SARS Spike S1 Protein (SAR-VM4S1) Summary
Product Description |
Recombinant SARS spike S1 protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser14-Arg667. |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated SARS Spike S1 Protein (SAR-VM4S1) Specifications/Details
Protein |
Spike S1 |
Synonyms |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Accession |
|
Species |
SARS |
Biotinylated |
no |
Amino Acid Range |
Ser14-Arg667 |
Molecular Weight |
The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
SARS Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of SARS spike S1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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