SARS Spike S1 Protein (SAR-VM5S1)
SARS Spike S1 Protein (SAR-VM5S1)
SARS Spike S1 Protein (SAR-VM5S1)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS Spike S1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser14-Arg667
Catalog #SAR-VM5S1
Regular price $300.00
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Product Description

Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser14-Arg667.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

Spike S1

Synonyms

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Accession

Species

SARS

Biotinylated

no

Amino Acid Range

Ser14-Arg667

Molecular Weight

The protein has a predicted MW of 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized SARS Spike S1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized SARS Spike S1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21ug/ml determined by ELISA. Immobilized SARS Spike S1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, His Tag with the EC50 of 0.21ug/ml determined by ELISA.
SARS Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-SARS spike S1 is greater than 95% as determined by SEC-HPLC. The purity of-SARS spike S1 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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