Biotinylated Human SEZ6 Protein (SEZ-HM406B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu20-His925

Catalog: SEZ-HM406B

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Product Description	Recombinant Biotinylated Human SEZ6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-His925.
Product Background	Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
Product Category	Recombinant Protein

Target	SEZ6
Gene/Protein ID	Q53EL9-1
Species	Human
Exact Sequence	Leu20-His925
Molecular Weight	The protein has a predicted MW of 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
	The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human SEZ6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.