Name: Biotinylated Human SEZ6 Protein (SEZ-HM406B)
Brand: KactusBio
SKU: SEZ-HM406B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human SEZ6 Protein (SEZ-HM406B)

Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).

Biotinylated Human SEZ6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-SEZ6 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human SEZ6, His-Avi Tag with an affinity constant of 3.31 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu20-His925

Catalog #SEZ-HM406B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human SEZ6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-His925.
Product Background	Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	SEZ6
Synonyms	SEZ-6; SEZ6; BSRPC; BSRP-C
Accession	Q53EL9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu20-His925
Molecular Weight	The protein has a predicted MW of 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human SEZ6, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human SEZ6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-SEZ6 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human SEZ6, His-Avi Tag with an affinity constant of 3.31 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human SEZ6 is greater than 95% as determined by SEC-HPLC.

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