- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Sus scrofa IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Val34-Asn190
Product Description |
Recombinant Sus scrofa IL-22 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val34-Asn190. |
Product Background |
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-22 |
Synonyms |
Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18 |
Accession |
|
Species |
Sus scrofa |
Biotinylated |
no |
Amino Acid Range |
Val34-Asn190 |
Molecular Weight |
The protein has a predicted MW of 19.77 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Sus scrofa IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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