• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD46, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Cys35-Asp329
Catalog #CD4-CM146
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus CD46 Protein (CD4-CM146) Summary

Product Description

 Recombinant Cynomolgus CD46 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys35-Asp329.

Product Background

 CD46 was discovered in 1986 during a search for novel C3b-binding proteins. CD46 is expressed ubiquitously and functions as a co-factor in the factor I-mediated proteolytic cleavage of C3b and C4b. Its vital role in preventing complement deposition on host tissue is underpinned by the fact that deficiency of CD46 is a predisposing factor for numerous disease conditions arising from complement-mediated 'self-attack'.

Cynomolgus CD46 Protein (CD4-CM146) Specifications/Details

Protein
 CD46
Synonyms
 TLX; CD46; MCP; MIC10; AHUS2; TRA2.10
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Cys35-Asp329
Molecular Weight
 The protein has a predicted MW of 34.05 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus CD46, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus CD46, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test). Immobilized Cynomolgus CD46, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.46ug/ml determined by ELISA (QC Test).
Cynomolgus CD46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus CD46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CD46 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus CD46 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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