Product Description |
Recombinant Biotinylated Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54. |
Product Background |
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF). |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
BCMA/TNFRSF17 |
Synonyms |
CD269; TNFRSF17; BCMA; BCM; TNFRSF13A |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Ala54 |
Molecular Weight |
The protein has a predicted MW of 8.9 kDa. Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human BCMA, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human BCMA, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). |
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Immobilized Human APRIL (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 38.2ng/ml determined by ELISA. |
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Immobilized Anti-BCMA Antibody, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. |
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Biotinylated Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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