• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Asp24-Ser529
Catalog #GPC-MM111
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Mouse Glypican 1/GPC1 Protein (GPC-MM111) Summary

Product Description

 Recombinant Mouse Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser529.

Product Background

 CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.

Mouse Glypican 1/GPC1 Protein (GPC-MM111) Specifications/Details

Protein
 Glypican 1/GPC1
Synonyms
 Glypican-1; FLJ38078; GPC1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Asp24-Ser529
Molecular Weight
 The protein has a predicted MW of 56.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 50mM Tris,150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Glypican 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Glypican 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Glypican 1 is greater than 95% as determined by SEC-HPLC. The purity of Mouse Glypican 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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