• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Catalog #ILR-HM2BG
Price
Regular price $37500
Regular price Sale price $37500
/
Size
Avantor VWR Fisher Scientific

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Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG) Summary

Product Description

 Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of IL-2 R beta and IL-2 R gamma.It contains Ala27-Thr240 (IL-2 R beta) and Leu23-Ala262 (IL-2 R gamma).

Product Background

 Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.

Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG) Specifications/Details

Protein
 IL-2 R beta&IL-2 R gamma
Synonyms
 IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ;IL2RB&IL2RG; IL2RG; IL2R subunit gamma; IL2R subunit beta; IL2RB; IL2R beta&IL2R gamma; IL2R subunit β; IL2R β&IL2R gamma; IL2Rγ; IL2R γ;IL-2R subunit b; IL2R subunit b; IL-2 R b&IL-2 R gamma; IL2R b&IL2R gamma; IL-2R gamma; IL2R gamma; IL-2R g; IL2R g; IL-2 Rg; IL-2 R gamma; IL-2 R g
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Molecular Weight
 The protein has a predicted MW of 51.3 kDa (IL-2 R beta) and 54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa and 80-100 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
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