• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly32-Ser349
Catalog #NEC-HM204
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Human Nectin-4 Protein (NEC-HM204) Summary

Product Description

 Recombinant Human Nectin-4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly32-Ser349.

Product Background

 NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets

Human Nectin-4 Protein (NEC-HM204) Specifications/Details

Protein
 Nectin-4
Synonyms
 EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly32-Ser349
Molecular Weight
 The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test).
Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Human Nectin-4 is greater than 95% as determined by SEC-HPLC. The purity of-Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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