• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln22-Val146
Catalog #RSP-HM103
Price
Regular price $26400
Regular price Sale price $26400
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Size
Avantor VWR Fisher Scientific

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Human R spondin 3/RSPO3 Protein (RSP-HM103) Summary

Product Description

 Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val146.

Product Background

 The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.

Human R spondin 3/RSPO3 Protein (RSP-HM103) Specifications/Details

Protein
 R spondin 3/RSPO3
Synonyms
 R-spondin-3; RSPO3; hPWTSR; hRspo3; PWTSR; THSD2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln22-Val146
Molecular Weight
 The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test). Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test).
Human R spondin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human R spondin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A). Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A).
Other Available Proteins:
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