• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln59-Ala404
Catalog #SIG-HM101
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Human CD209/DC-SIGN Protein (SIG-HM101) Summary

Product Description

 Recombinant Human CD209/DC-SIGN Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln59-Ala404.

Product Background

 C-type lectin CD209/DC-SIGN and CD209L/L-SIGN proteins are distinct cell adhesion and pathogen recognition receptors that mediate cellular interactions and recognize a wide range of pathogens, including viruses such as SARS, SARS-CoV-2, bacteria, fungi and parasites. Pathogens exploit CD209 family proteins to promote infection and evade the immune recognition system.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD209/DC-SIGN Protein (SIG-HM101) Specifications/Details

Protein
 CD209/DC-SIGN
Synonyms
 CD209; CLEC4L; DC-SIGN; DC-SIGN1; CDSIGN; MGC129965; CD209 molecule
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln59-Ala404
Molecular Weight
 The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Product Tag
 N-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Human CD209 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD209 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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