• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val114-Gly281
Catalog #TRL-HE001
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001) Summary

Product Description

 Recombinant Human TRAIL/TNFSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.

Product Background

 Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.

Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001) Specifications/Details

Protein
 TRAIL/TNFSF10
Synonyms
 Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2;Apo2 ligand
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val114-Gly281
Molecular Weight
 The protein has a predicted MW of 19.6 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM Tris, 300mM NaCl (pH 7.2). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).
Immobilized Human TRAIL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. Immobilized Human TRAIL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Human TRAIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TRAIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TRAIL is greater than 95% and the molecular weight of this protein is around 45-65 kDa as determined by SEC-MALS. The purity of Human TRAIL is greater than 95% and the molecular weight of this protein is around 45-65 kDa as determined by SEC-MALS.
The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC. The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...